Located in Darmstadt-Kranichstein, the Eisenbahn Museum occupies a railroad depot established in 1898. The museum has been open for over 30 years and has over 40 locomotives and more than 150 carriages in its collection.

The Museum features working steam and diesel locomotives, a large model train layout, historical vehicles and displays, and frequently-scheduled steam train excursions. Historic working steam engines include still-operational DRG 23 042, 01 1056, 41 024, and 44 404 steam engines, steam locomotive 4981 "Mainz" (a Prussian G 8), which was transferred from Turkey, the Austrian locomotive 97 210, a rack railway steam engine from the Erzberg railway, the DB locomotive number 103 101-2, the DRG E 16 08 electric engine, and several DB Class V 36 diesel locomotives.

The museum operates steam excursions monthly in conjunction with the Deutsch Bundesbahn.